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Other Democratic lawmakers have proposed abortion-related bills as well. "I don't want any confusion and I don't want a woman being prosecuted for getting an abortion or being suspected of getting an abortion," he continued. While the state's most recent law does not allow that, Johnson said he wants to eliminate any possibility of that happening. Jack spoke with Johnson this week, who said he's focused on his re-election bid and not on potential challengers. Hinojosa, who left his job last July earlier than planned so he could consider a run, said he decided against challenging Mayor Eric Johnson because the timing isn't ideal, his consultant work is keeping him very busy and that it would be difficult to campaign for the job. Michael Hinojosa told CBS 11 he has decided not to run. ![]() It's still months away, but the field of potential candidates in the 2023 Dallas Mayor's race has already narrowed. A look ahead to the race for Dallas mayor Watch the latest episode of Eye on Politics in the video player above and stream new episodes live every Thursday and Friday at 7 p.m. 8).Įvery week, CBS 11 political reporter Jack Fink breaks down some of the biggest political stories grabbing headlines in North Texas and beyond. All these stories and more in this week's edition of Eye on Politics (original air date: Dec. Michael Hinojosa told Jack he won't run for mayor. ![]() And Dallas Mayor Eric Johnson spoke with Jack Fink about his re-election bid after former Dallas ISD Superintendent Dr. Texas lawmakers have filed bills to increase and repeal abortion restrictions for the upcoming legislative session. NORTH TEXAS (CBSDFW.COM) - History was made in Fort Worth this week. A "Maintenance of Certification Program" encourages board certified physicians to continue learning and self-evaluating throughout their medical career.Eye on Politics: The race for Mayor of Dallas, history made in Fort Worth 15:44
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We’ve talked a lot about the quality control process at Addgene by introducing our new sequencing partner seqWell and going into detail about how we use next generation sequencing results to perform quality control on deposited plasmids.Gene cloning is customarily required to study the gene functions in vivo and in vitro. We’ve also talked about how our new Snapgene generated maps provide improved feature detection with an easy to use interface. We regularly use Snapgene for our quality control process because of its expansive feature library and useful tools. To design experiments for further plasmid verification.To align NGS sequencing results with reference sequences.The main ways we use SnapGene for plasmid quality control are: In this blog, we’ll walk you through how a scientist at Addgene uses Snapgene to confirm the sequence of a plasmid and we’ll highlight some of the new features available on our website through our Snapgene powered maps and sequence analysis tools. While our quality control process for incoming plasmids can vary based on the contents of a plasmid, the first step is always to send prepped plasmid DNA for next generation sequencing. We then copy the contents of the resulting FASTA file into the desktop version of Snapgene to visualize the sequence and look for features. The first thing that the Snapgene software will confirm for us is whether it is a linear or circular sequence. Next, we use the “Detect Common Features” function to find and annotate promoters, selectable markers, tags, origins of replication, and fluorescent proteins that are already included in Snapgene’s extensive database. Snapgene also provides easy visualization of open reading frames, which is often our first clue about where the gene of interest might be located. By easily using BLAST to check the translated amino acid sequence from within Snapgene, we can confirm the identity of the gene and check for any mutations. Once we identify the gene, we can easily annotate it by making a custom feature in Snapgene. This saves us time as we are often analyzing deposits with many plasmids that contain similar features and it allows you to more quickly identify the same feature in other plasmids. Once we’ve identified the features within a plasmid, we next verify that our sequencing results match sequence information contributed by the depositor, published backbone sequences, and any known sequences for the insert. The Snapgene desktop software allows us to quickly align multiple sequences at the same time. Using Snapgene for additional verification experiments: Primer and restriction digest design This means we can easily compare and analyze any differences between our sequence results and the reference sequences. Some regions of plasmids are difficult to sequence by NGS. These can include GC-rich and repetitive regions. These regions require additional verification experiments that can be partially designed using the Snapgene desktop software. If a plasmid contains a region with a high amount of guanine and cytosine nucleotides, we might need to send it for Sanger sequencing to verify the sequence within that region. We have designed many primers for Sanger sequencing at Addgene. By using Snapgene’s “Import primers from a list” function, we can detect all of the primers we have designed that will anneal to the plasmid sequence. ![]() ![]() But don’t let the fake photoshopped image of the two actors sweetly kissing above fool ya: it wasn’t all lovey-dovey while it lasted. Dean apparently “stayed after the class to meet Brando, which lead to a sexual affair that lasted for several months,” according to HomoHistory. The two star hunks met when Brando taught a class at Dean’s acting school. Perhaps James Dean’s biggest sexual conquest (allegedly!) was fellow actor Marlon Brando. Bast claimed he “experimented” with Dean and that things sometimes got sexual. Once in New York, James Dean found a roommate and close friend in William Bast, who revealed later in life that his relationship with Dean was more than a platonic bromance. The pair remained friends even after Dean moved out and became a star. Later Brackett even paid for Dean to move to New York City so he could study acting, and he introduced Dean to the director of his first movie, East of Eden. “It was a classic ‘kept boy’ scenario,” according to HomoHistory. ![]() apartment with Rogers Brackett, a 35-year-old radio director and producer who bought him nice clothes, took him out to fancy dinners and introduced him to Hollywood heavyweights to help kickstart Dean’s career. Here, then, is the alleged list of Dean’s conquests.Ī struggling actor at the time, 20-year-old James Dean shared an L.A. ![]() Even his close friend, Elizabeth Taylor, once said matter-of-factly that all her best friends had been gay, including Montgomery Cliff, Rock Hudson and the one and only Jimmy Dean. “There’s been quite an evolution in the thinking since Dean’s death in 1955,” biographer Val Holley said, “moving from ‘James Dean was straight’ to ‘Dean had sex with men but only to advance his career’ to ‘Dean had sex with women but only to advance his career.’”īoth the director and screenwriter of Rebel without a Cause believed James Dean was exclusively gay. ![]() That an actor is gay or bi-sexually isn’t at all hard to believe, but when it conflicts with the on screen image, the public chooses to reject the truth. Most of the accounts from those who were his friends and associates show that he had “very close” relationships with several gay and bi-sexual men. At a time when it was impossible for male leads to be openly gay, it wasn’t at all unusual for gay actors like Rock Hudson to enter into sham marriages.īoth the gay and straight communities want to claim Dean as one of their own, but I think it’s safe to say that he was gay. Angeli abruptly broke off that engagement which led to further speculation. Rumors about Dean’s sexuality began to spread as his fame increased and he quickly became engaged to the actress Pier Angeli. But, I’m also not going to go through life with one hand tied behind my back.” Later, when questioned if he was homosexual he is reported to have said, “No, I am not a homosexual. James Dean avoided the draft by registering as a homosexual. ![]() There are conflicting reports about Dean’s sexuality there are a few who try to deny that he had any sexual affairs with men, there are those who say that he was bisexual and some who say that he was exclusively gay (those include Rebel without a Cause director Nicholas Ray and screenwriter Gavin Lambert.)ĭean publicly dated women and privately dated men such as the actors Clifton Webb, Brando, Jack Simmons, Bill Bast as well as the producer Rogers Brackett. Was he or wasn’t he? I think we’d all like to BELIEVE that James Dean was gay – or at least bisexual – and that if we were around at the time we’d have a shot with him. According to HomoHistory: ![]() |
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